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pgm t cloning kit  (tiangen biotech co)


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    Structured Review

    tiangen biotech co pgm t cloning kit
    Pgm T Cloning Kit, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 96/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgm+t+blunt+cloning+kit/pGM-T+Blunt+Cloning+Kit/pm41528528-67-16-19
    Average 96 stars, based on 101 article reviews
    pgm t cloning kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cloning:

    Article Title: Simulated Climate Warming Influenced Colony Microclimatic Conditions and Gut Bacterial Abundance of Honeybee Subspecies <i>Apis mellifera ligustica</i> and <i>A. mellifera sinisxinyuan</i>
    Article Snippet: To this end, amplified target gene fragments were cloned in Top10® (Escherichia coli) Competent Cells (Tiangen®, Beijing, P.R. .. China) using pGM-T Blunt Cloning Kit (Tiangen®, Beijing, P.R. ..

    Article Title: Tandem Repeat Generation and Novel Isothermal Amplification Based on Nonspecific Tailing and Replication Slippage.
    Article Snippet: After electrophoresis, the gel was stained with GelRed and visualized by the ChemiDoct XRS Imaging System (Bio-Rad Laboratories Co., Ltd.). .. The Bst-mediated nonspecific products were first ligated into a pGM-T Vector by using pGM-T Blunt Cloning Kit (TIANGEN Biotech) according to the manufacturer’s protocol. .. Then, pGM-T Vectors were transformed into competent cells, which were smeared on solid lysogeny broth plates containing antibiotics and cultured at 37°C for 12 to 16 h. Then, the colonies were respectively transformed into liquid lysogeny broth culture medium (containing 100 μg/ml ampicillin) and cultured at 37°C for 12 to 16 h. The plasmid DNAs were purified from the enlarged colonies, followed by Sanger sequencing analysis by Sangon Biotech by using M13F (5’-caggaaacagctatgacc-3’) and M13R (5’-tgtaaaacgacggccagt-3’) as sequencing primers.

    Article Title: Cloning and Characterization of Recombinant Glutamate Decarboxylase from Coffea arabica Leaves: Structural Insights and Ultrasound-Induced Modifications.
    Article Snippet: Gamma-aminobutyric acid (GABA), a nonprotein amino acid with diverse functions, is synthesized by glutamate decarboxylase (GAD).. While ultrasound has been shown to affect GAD activity, the underlying mechanism remains unclear.. In this study, we successfully engineered a recombinantEscherichia coli (E. coli) strain carrying the Coffea arabica GAD (CaGAD) gene for the first time and characterized the bioinformatics characteristics of CaGAD.

    Plasmid Preparation:

    Article Title: Cloning and Characterization of Recombinant Glutamate Decarboxylase from Coffea arabica Leaves: Structural Insights and Ultrasound-Induced Modifications.
    Article Snippet: Gamma-aminobutyric acid (GABA), a nonprotein amino acid with diverse functions, is synthesized by glutamate decarboxylase (GAD).. While ultrasound has been shown to affect GAD activity, the underlying mechanism remains unclear.. In this study, we successfully engineered a recombinantEscherichia coli (E. coli) strain carrying the Coffea arabica GAD (CaGAD) gene for the first time and characterized the bioinformatics characteristics of CaGAD.



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    tiangen biotech co pgm t cloning kit
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